Recombinant Human MCM8 protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： Q9UJA3-1
| 别称 |
DNA helicase MCM8;MCM8;Minichromosome maintenance 8
|
| 表达系统 |
E.coli
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| 序列 |
Met1-Ser250
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| 蛋白编码 |
Q9UJA3-1
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| 种属 |
Human
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| 计算分子量 |
27.4 kDa
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| 表观分子量 |
35 kDa
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| 标签 |
N-His & C-His
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| 生物活性 |
Not validated for activity
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| 纯度 |
> 95% as determined by reducing SDS-PAGE.
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| 内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
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| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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| 背景 |
Component of the MCM8-MCM9 complex, a complex involved in homologous recombination repair following DNA interstrand cross-links and plays a key role during gametogenesis. The MCM8-MCM9 complex probably acts as a hexameric helicase downstream of the Fanconi anemia proteins BRCA2 and RAD51 and is required to process aberrant forks into homologous recombination substrates and to orchestrate homologous recombination with resection, fork stabilization and fork restart.
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实验操作视频
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