Recombinant Human MeCP2 protein(His Tag)

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- 表达系统: E.coli
- 蛋白编码: P51608
别称 |
MeCp-2 protein
|
表达系统 |
E.coli
|
序列 |
Met1-Lys177
|
蛋白编码 |
P51608
|
种属 |
Human
|
计算分子量 |
19.0 kDa
|
表观分子量 |
32 kDa
|
标签 |
N-His
|
生物活性 |
Not validated for activity
|
纯度 |
> 95% as determined by reducing SDS-PAGE.
|
内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
|
保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
|
复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
|
背景 |
Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).
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实验操作视频
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