Recombinant Human MMP-9 protein (His Tag)
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- 表达系统： HEK293 Cells
- 蛋白编码： P14780
| 别称 |
92 kDa gelatinase;92 kDa type IV collagenase;CLG4B;GELB;Gelatinase B;MANDP2;MMP9;Matrix metalloproteinase-9
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| 表达系统 |
HEK293 Cells
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| 序列 |
Met1-Asp707
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| 蛋白编码 |
P14780
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| 种属 |
Human
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| 计算分子量 |
77.7 kDa
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| 表观分子量 |
85 kDa
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| 标签 |
C-His
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| 生物活性 |
Not validated for activity
|
| 纯度 |
> 90% as determined by reducing SDS-PAGE.
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| 内毒素 |
< 1.0 EU/mg of the protein as determined by the LAL method
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
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| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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| 背景 |
Matrix metallopeptidase 9 (MMP-9) is an enzyme encoded by the MMP9 gene. This protein, which is produced by normal alveolar macrophages and granulocytes, can be activated by 4-aminophenylmercuric acetate and phorbol ester and up-regulated by ARHGEF4, SPATA13 and APC via the JNK signaling pathway in colorectal tumor cells. MMP-9 is involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, angiogenesis, bone development, wound healing, cell migration, learning and memory, as well as in pathological processes, such as arthritis, intracerebral hemorrhage, and metastasis.
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实验操作视频
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