Recombinant Human MPO Protein(Trx Tag)
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- 表达系统： E.coli
- 蛋白编码： P05164
| 别称 |
fj80f;wu:fj80f;MPO;84 kDa myeloperoxidase;89 kDa myeloperoxidase;EC 1.11.1.7;EC1.11.2.2;fj80f04;mpx;myeloid-specific peroxidase;Myeloperoxidase;Myeloperoxidase heavy chain;Myeloperoxidase light chain;PERM;wu:fj80f04;84 kDa myeloperoxidase;Myeloperoxidase;84 kDa myeloperoxidase;89 kDa myeloperoxidase;EC 1.11.1.7;EC1.11.2.2;fj80f04;MPO;mpx;myeloid-specific peroxidase;Myeloperoxidase;Myeloperoxidase heavy chain;Myeloperoxidase light chain;PERM;wu:fj80f04
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| 表达系统 |
E.coli
|
| 序列 |
Cys606-Ser745
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| 蛋白编码 |
P05164
|
| 种属 |
Human
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| 计算分子量 |
35.2 kDa
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| 表观分子量 |
35 kDa
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| 标签 |
N-Trx
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| 生物活性 |
Not validated for activity
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| 纯度 |
> 90% as determined by reducing SDS-PAGE.
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| 内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
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| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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| 背景 |
Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor , myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils.
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实验操作视频
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