NBL;DAND;DAN;DAND1;NBL1;D1S1733E;DAN domain family member 1;DAND 1;Differential screening selected gene aberrant in neuroblastoma;MGC8972;NBL 1;Neuroblastoma candidate region suppression of tumorigenicity 1;Neuroblastoma suppression of tumorigenicity 1;Neuroblastoma suppressor of tumorigenicity 1;NO 3;NO3;Zinc finger protein DAN

Mammalian

Ala17-Asp181

P41271

Human

43.1 kDa

55 kDa

C-Fc

Not validated for activity

> 90% as determined by reducing SDS-PAGE.

< 1.0 EU/mg of the protein as determined by the LAL method

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

This product is provided as lyophilized powder which is shipped with ice packs.

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

The Dan (Differential screening-selected gene aberrative in neuroblastoma , also known as N03) gene was first identified as the putative rat tumor suppressor gene and encodes a protein structurally related to Cerberus and Gremlin in the vertebrates. It is a founding member of the DAN family of secreted proteins , acts as an inhibitor of cell cycle progression , and is closely involved in retinoic acid-induced neuroblastoma differentiation. There are at least five mammalian protein members in the evolutionarily conserved Dan family including DAN , Gremlin/DRM , Cer1 (Cerberus-related) , Dante , and PRDC (protein related to DAN and Cerberus) , and share the C-terminal cystine-knot motif. As a secreted glycoprotein , DAN is a member of a class of glycoproteins shown to be secreted inhibitors of the transforming growth factor-beta (TGF-beta) and bone morphogenic protein pathways. It binds to BMPs and preventing their interactions with signaling receptor complexes , and accordingly regulates the processes of embryonic development and tissue differentiation. DAN gene product may have an important role in the regulation of the entry of cells into the S phase. Besides , the DAN gene product possesses an ability to revert phenotypes of transformed rat fibroblasts and represents a candidate tumor suppressor gene for neuroblastoma.