Recombinant Human PD-1/PDCD1 Protein (His Tag)
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- 表达系统： HEK293 Cells
- 蛋白编码： Q15116
- 应用性： ELISA
| 别称 |
CD279;Hsle1;PD-1;PDCD1;Programmed cell death protein 1;SLEB2;hPD-1
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| 表达系统 |
HEK293 Cells
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| 序列 |
Pro21-Gln167
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| 蛋白编码 |
Q15116
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| 种属 |
Human
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| 计算分子量 |
17 kDa
|
| 表观分子量 |
30-42 kDa
|
| 标签 |
C-His
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| 生物活性 |
Immobilized Human PD-1-His at 2μg/ml (100 μl/well) can bind Anti-PD1 mAb. The ED50 of Anti-PD1 mAb is 9.74 ng/ml.
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 10% Trehalose, 100mM NaCl, 0.05% Tween 80, pH 7.5.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
Programmed cell death protein 1(PDCD1) is a single-pass type I membrane protein and contains 1 Ig-like V-type domain. PD-1 is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 inhibits the T-cell proliferation and production of related cytokines including IL-1; IL-4; IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition; coligation of PDCD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PDCD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance; and thus contributes to the prevention of autoimmune diseases. As a cell surface molecule; PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation; cytokine production; and cytolytic function.
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实验操作视频
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