Recombinant Human PDCD4 protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： Q53EL6
- 应用性： Immunogen
| 别称 |
Death up-regulated gene protein;Dug;H731;MGC33046;MGC33047;Ma3;Neoplastic transformation inhibitor;Neoplastic transformation inhibitor protein;Nuclear antigen H731;Nuclear antigen H731 like;Nuclear antigen H731 like protein;Nuclear antigen H731-like;PDCD
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| 表达系统 |
E.coli
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| 序列 |
Met1-Asn325
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| 蛋白编码 |
Q53EL6
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| 种属 |
Human
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| 计算分子量 |
36.9 kDa
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| 表观分子量 |
41-43 kDa
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| 标签 |
N-His
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| 生物活性 |
Not validated for activity
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| 纯度 |
> 90% as determined by reducing SDS-PAGE.
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| 内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
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| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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| 背景 |
Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation.
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实验操作视频
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