Recombinant Human PDGF-AA Protein
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- 表达系统： E.coli
- 蛋白编码： P04085
| 别称 |
PDGF subunit A;PDGF-1;PDGF1;PDGFA;Platelet-derived growth factor A chain;Platelet-derived growth factor alpha polypeptide;Platelet-derived growth factor subunit A
|
| 表达系统 |
E.coli
|
| 序列 |
Ser87-Thr 211
|
| 蛋白编码 |
P04085
|
| 种属 |
Human
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| 计算分子量 |
14.4 kDa
|
| 表观分子量 |
16 kDa
|
| 标签 |
None
|
| 生物活性 |
Not validated for activity
|
| 纯度 |
> 90 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 20mM Glycine-HCl, 6% Sucrose, 4% Mannitol, 0.02% Tween 80, pH 3.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Platelet-derived growth factor subunit A (PDGFA); belongs to the PDGF/VEGF growth factor family. PDGFA is a secreted protein; stored in platelet alpha-granules and released by platelets upon wounding. PDGFA is potent mitogens for a variety of cell types including smooth muscle cells; connective tissue cells; bone and cartilage cells; and some blood cells. It plays an essential role in the regulation of embryonic development; cell proliferation; cell migration; survival and chemotaxis. PDGFA is required for normal lung alveolar septum formation during embryogenesis; normal development of the gastrointestinal tract; normal development of Leydig cells and spermatogenesis; normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. It plays an important role in wound healing; Signaling is modulated by the formation of heterodimers with PDGFB.
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实验操作视频
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