Recombinant Human PIK3CA Protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： P42336-1
| 别称 |
5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha;5-bisphosphate 3-kinase catalytic subunit alpha isoform;CLOVE;CWS5;MCAP;MCM;MCMTC;MGC142161;MGC142163;Phosphatidy;Phosphatidylinositol 3 kinase catalytic alpha polypeptide;caPI3K;p110 alpha;p110alpha
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| 表达系统 |
E.coli
|
| 序列 |
Met1-Pro200
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| 蛋白编码 |
P42336-1
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| 种属 |
Human
|
| 计算分子量 |
23.1 kDa
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| 表观分子量 |
29 kDa
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| 标签 |
N-His
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| 生物活性 |
Not validated for activity
|
| 纯度 |
> 95% as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
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| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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| 背景 |
Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22.
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实验操作视频
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