Recombinant Human PL/PNLIP protein (His Tag)
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- 表达系统： HEK293 Cells
- 蛋白编码： P16233
| 别称 |
PL) (PTL) (Pancreatic lipase) (EC 3.1.1.3);Pancreatic triacylglycerol lipase
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| 表达系统 |
HEK293 Cells
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| 序列 |
Met1-Cys465
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| 蛋白编码 |
P16233
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| 种属 |
Human
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| 计算分子量 |
51.0 kDa
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| 表观分子量 |
50 kDa
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| 标签 |
C-His
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| 生物活性 |
Not validated for activity
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| 纯度 |
> 95% as determined by reducing SDS-PAGE.
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| 内毒素 |
< 1.0 EU/mg of the protein as determined by the LAL method
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
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| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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| 背景 |
PNLIP is an enzyme which belongs to the lipase family. Secreted from the pancreas, PNLIP is the primary lipase that hydrolyzes dietary fat molecules in the human digestive system, converting triglyceride substrates found in ingested oils to monoglycerides and free fatty acids. Bile salts secreted from the liver and stored in gallbladder are released into the duodenum where they coat and emulsify large fat droplets into smaller droplets, thus increasing the overall surface area of the fat, which allows the lipase to break apart the fat more effectively. The resulting monomers (2 free fatty acids and one 2-monoacylglycerol) are then moved by way of peristalsis along the small intestine to be absorbed into the lymphatic system by a specialized vessel called a lacteal.
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实验操作视频
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