Recombinant Human PTEN Protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： P60484-1
| 别称 |
10q23del;BZS;DEC;GLM2;MGC11227;MHAM;MMAC1;MMAC1 phosphatase and tensin homolog deleted on chromosome 10;Mutated in multiple advanced cancers 1;Phosphatase and tensin homolog;Phosphatase and tensin like protein;Phosphatidylinositol 3,4,5-trisphosphate 3-ph
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| 表达系统 |
E.coli
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| 序列 |
Thr2-Val403
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| 蛋白编码 |
P60484-1
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| 种属 |
Human
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| 计算分子量 |
46.8 kDa
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| 表观分子量 |
49.1 kDa
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| 标签 |
N-His
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| 生物活性 |
Not validated for activity
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| 纯度 |
> 85% as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
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| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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| 背景 |
PTEN (also designated MMAC1),products of tumor suppressor genes,are found deleted in most human gliomas. The PTEN genes are also mutated in many other tumors,such as brain,breast,kidney and prostate cancers. PTEN is a protein tyrosine phosphatase that may terminate the signaling transduction pathways mediated by PI 3-kinase/Akt. PTEN has an apparent molecular weight of 55 kDa and it is located in the cytosol.
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实验操作视频
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