Recombinant Human Ribonuclease 3/RNASE3 Protein (His Tag)
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- 表达系统： HEK293 Cells
- 蛋白编码： AAH96060.1
| 别称 |
ECP;Eosinophil Cationic Protein;RNASE3;RNS3;RNase 3;Ribonuclease 3
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| 表达系统 |
HEK293 Cells
|
| 序列 |
Arg28-Ile160
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| 蛋白编码 |
AAH96060.1
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| 种属 |
Human
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| 计算分子量 |
16.6 kDa
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| 表观分子量 |
25-35 kDa
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| 标签 |
C-His
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| 生物活性 |
Not validated for activity
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
|
| 运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
|
| 制剂 |
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mMDTT, 10% Glycerol, pH 7.5.
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| 复溶方法 |
Not Applicable
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| 背景 |
Ribonuclease 3 (RNASE3) is a basic protein that is localized to the eosinophil primary matrix and belongs to the pancreatic ribonuclease family. RNASE3 is released during degranulation of eosinophils. RNASE3 possesses a wide variety of biological activities. RNASE3 interacts with bacterial lipopolysaccharide (LPS) and lipoteichoic acid (LTA). RNASE3 exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. It promotes E. coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.
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实验操作视频
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