Recombinant Human RNASE3 Protein(His Tag)
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- 表达系统： Mammalian
- 蛋白编码： P12724
| 别称 |
RAF;RNS;Ribonuclease;RNASE3;ECP;RAF1;RNS3;Eosinophil cationic protein;Ribonuclease 3;Rnase 3;Ribonuclease A family member 3
|
| 表达系统 |
Mammalian
|
| 序列 |
Met1-lle160
|
| 蛋白编码 |
P12724
|
| 种属 |
Human
|
| 计算分子量 |
17.5 kDa
|
| 表观分子量 |
25-30 kDa
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| 标签 |
C-His
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| 生物活性 |
Not validated for activity
|
| 纯度 |
> 95% as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU/mg of the protein as determined by the LAL method
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
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| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis
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| 背景 |
Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.
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实验操作视频
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