Recombinant Human S100A1 Protein(Trx Tag)

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- 表达系统: E.coli
- 蛋白编码: P23297
别称 |
Bpb;NEF;Protein S100-A1;S-100 protein alpha chain;S-100 protein subunit alpha;S100 alpha;S100 beta;S100 calcium binding protein A1;S100 calcium binding protein B;S100 calcium-binding protein A1;S100 protein alpha polypeptide;S100A;s100a1;S100B;S100beta;S10A1
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表达系统 |
E.coli
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序列 |
Met1-Ser94
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蛋白编码 |
P23297
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种属 |
Human
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计算分子量 |
30.3 kDa
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表观分子量 |
30 kDa
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标签 |
N-Trx
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生物活性 |
Not validated for activity
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纯度 |
> 90% as determined by reducing SDS-PAGE.
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内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
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保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
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复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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背景 |
Small calcium binding protein that plays important roles in several biological processes such as Ca2+ homeostasis, chondrocyte biology and cardiomyocyte regulation (PubMed:12804600). In response to an increase in intracellular Ca2+ levels, binds calcium which triggers conformational changes (PubMed:23351007). These changes allow interactions with specific target proteins and modulate their activity (PubMed:22399290). Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca2+ cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca2+-ATPase/ATP2A2 and mitochondrial F1-ATPase (PubMed:12804600). Facilitates diastolic Ca2+ dissociation and myofilament mechanics in order to improve relaxation during diastole.
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实验操作视频
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