Recombinant Human S100A8 Protein(GST Tag)
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- 表达系统： E.coli
- 蛋白编码： P05109
| 别称 |
Protein S100-A;S100a;Kcnip;KCHIP;MRP;S100A8;60B8AG;CAGA;CFAG;CGLA;CP-10;L1Ag;MA387;MIF;MRP8;NIF;P8;Calgranulin A;Calgranulin-A;Cystic fibrosis antigen;Leukocyte L1 complex light chain;MRP-8;Protein S100-A8;CALP;KCNIP4;KCHIP4;AI323541;B8Ag;BEE11;Calprotectin;Calprotectin L1L subunit;Chemotactic cytokine CP-10;included;Migration inhibitory factor-related protein 8;Myeloid-related protein 8;Neutrophil cytosolic 7 kDa protein;Pro-inflammatory S100 cytokine;S100 calcium binding protein A8;S100 calcium binding protein A8 (calgranulin A);S100 calcium-binding protein A8;S100A8/S100A9 complex;S10A8;Urinary stone protein band A
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| 表达系统 |
E.coli
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| 序列 |
Met1-Glu93
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| 蛋白编码 |
P05109
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| 种属 |
Human
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| 计算分子量 |
36.2 kDa
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| 表观分子量 |
40 kDa
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| 标签 |
N-GST
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| 生物活性 |
Not validated for activity
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| 纯度 |
> 95% as determined by reducing SDS-PAGE.
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| 内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
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| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis
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| 背景 |
S100A8/S100A9 Heterodimer;S100A8 (also MRP8 and calgranulin A) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is produced by neutrophils and monocytes, and forms Ca2+-dependent heterodimer/heterotetramer complexes (termed calprotectin) with S100A9. It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. Human S100A8 is 93 amino acids (aa) in length. It contains two EF-hand motifs (aa 12 - 47 and 46 - 81) and one high-affinity Ca2+-binding site (aa 59 - 70). There may be one splice form that shows a 15 aa substitution for the C-terminal 14 amino acids. Although mouse S100A8 is cleaved by MMP-2 after Asn21, it is unclear if human S100A8 is susceptible. Full-length human S100A8 is 57% and 74% aa identical to mouse and canine S100A8, respectively.
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实验操作视频
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