Recombinant Human SEMA3A Protein(Fc Tag)
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- 表达系统： Mammalian
- 蛋白编码： Q14563
| 别称 |
SEMA3A;COLL1;HH16;Hsema-I;Hsema-III;SEMA1;SEMAD;SEMAIII;SEMAL;SemD;coll-1
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| 表达系统 |
Mammalian
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| 序列 |
Lys26-Phe546
|
| 蛋白编码 |
Q14563
|
| 种属 |
Human
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| 计算分子量 |
82.2 kDa
|
| 表观分子量 |
80-90 kDa
|
| 标签 |
C-Fc
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| 生物活性 |
Not validated for activity
|
| 纯度 |
> 90% as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU/mg of the protein as determined by the LAL method
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
|
| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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| 背景 |
This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with an schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with an the progression of Alzheimer's disease.
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实验操作视频
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