Recombinant Human SEMA4B Protein (His & Fc Tag)
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- 表达系统： HEK293 Cells
- 蛋白编码： Q9NPR2
| 别称 |
KIAA1745;SEMA4B;SEMAC;Semaphorin-4B
|
| 表达系统 |
HEK293 Cells
|
| 序列 |
Leu39-Glu712
|
| 蛋白编码 |
Q9NPR2
|
| 种属 |
Human
|
| 计算分子量 |
102.0 kDa
|
| 表观分子量 |
114 kDa
|
| 标签 |
C-Fc-His
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| 生物活性 |
Not validated for activity
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Semaphorin-4B is a single-pass type I membrane protein. SEMA4B is a member of the semaphorin family. The class 4 semaphorins are integral membrane proteins that are widely expressed throughout the nervous system. SEMA4B is a single-pass type I membrane protein and contains one Ig-like C2-type (immunoglobulin-like) domain; one PSI domain and one Sema domain. Human SEMA4B is expressed in neurons. SEMA4B inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. SEMA4B negatively regulates basophil-mediated Th2 and humoral memory responses.
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实验操作视频
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