Recombinant Human Signal Transducer CD24/CD24 (C-Fc)（PKSH033968）|-Elabscience伊莱瑞特【官网】

Recombinant Human Signal Transducer CD24/CD24 (C-Fc)

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货号：PKSH033968
说明书

说明书

Recombinant Human Signal Transducer CD24/CD24 (C-Fc)

Price: ¥3066.00 ¥966.00

Size:

50 μg 10 μg

表达系统： HEK293 Cells
蛋白编码： P25063
应用性： ELISA

别称	CD24;CD24A;Signal transducer CD24;Small cell lung carcinoma cluster 4 antigen
表达系统	HEK293 Cells
序列	Ser27-Gly 59
蛋白编码	P25063
种属	Human
计算分子量	29.8 kDa
表观分子量	33-50 kDa
标签	C-Fc
生物活性	Immobilized Anti-Human CD24 mAb at 2μg/ml (100 μl/well) can bind Human CD24-Fc: Biotinylated by NHS-biotin prior to testing. The ED50 of Human CD24-Fc is 7.62 ng/ml.
纯度	> 95 % as determined by reducing SDS-PAGE.
内毒素	< 1.0 EU per μg of the protein as determined by the LAL method.
保存条件	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
运输条件	This product is provided as lyophilized powder which is shipped with ice packs.
制剂	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
复溶方法	Please refer to the printed manual for detailed information.
背景	Signal Transducer CD24 is a heavily and variably glycosylated GPI-linked sialoprotein. Human CD24 is expressed on B lineage cells and granulocytes, on epithelial, neuronal, and muscle cells, and on a range of tumor cells. CD24 expression is regulated during lineage development and with the activation of various cell types. Antibody crosslinking of CD24 enhances the induction of apoptosis in B and T lymphocytes which contributes to negative selection and the induction of immune tolerance. CD24 on antigen presenting cells cooperates with B7 molecules in the costimulation of T cells. CD24 associates in cis with Siglec10 and with the danger-associated molecules HMGB1, HSP70, or HSP90 which are released from necrotic or damaged cells. Formation of these ternary complexes fills a protective role: the resulting Siglec10 signaling inhibits inflammatory responses that are otherwise induced by extracellular DAMPs.

> 95 % as determined by reducing SDS-PAGE.

Immobilized Anti-Human CD24 mAb at 2μg/ml (100 μl/well) can bind Human CD24-Fc: Biotinylated by NHS-biotin prior to testing. The ED50 of Human CD24-Fc is 7.62 ng/ml.

Q1:重组蛋白有活性吗？可以做活性检测吗？

活性说明的见说明书bio-activity部分；如果说明书显示没有生物活性或则显示生物活性检测中，则说明这个蛋白没有生物活性数据，需要客户自己检测。

Q2:重组蛋白是否测序验证过？

在售重组蛋白默认不做测序分析/验证。

Q3:蛋白的运输条件是什么？

我们的蛋白有液体和冻干粉2种形式，一般来说冻干粉可以用冰袋、蓝冰、干冰运输；液体可以用蓝冰或干冰运输。

Q4:蛋白是通过什么方式纯化的？

一般通过纯化标签进行亲和纯化，常规有镍柱/His Tag、protein AG/Fc tag、标签抗体凝胶等。

Q5:蛋白可以用于细胞培养/注射小鼠吗？

内毒素含量小于0.1 ng/μg (1 EU/μg)的蛋白都可以用于细胞培养/注射小鼠。

Q6:蛋白发货形式可以是冻干粉吗？可以发液体吗？

默认按照说明书标注的形式发货；如果有特殊需求，需要根据蛋白情况确定。原则上，冻干粉蛋白可以发液体形式，液体蛋白不能发冻干粉形式。