Recombinant Human TARC Protein(Trx Tag)

Price:
- 表达系统: E.coli
- 蛋白编码: Q92583
别称 |
A-152E5.3;A152E53;ABCD 2;ABCD2;C-C motif chemokine 17;CC chemokine TARC;CCL17;CCL17;Chemokine CC Motif Ligand 17;MGC138271;MGC138273;SCYA17;Small Inducible Cytokine A17;Small Inducible Cytokine A17 Precursor;Small Inducible Cytokine Subfamily A (Cys Cys);Small Inducible Cytokine Subfamily A (Cys Cys) Member 17;Small-inducible cytokine A17;T Cell Directed CC Chemokine;Thymus and activation regulated chemokine;Thymus and activation-regulated chemokine
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表达系统 |
E.coli
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序列 |
Ala24-Ser94
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蛋白编码 |
Q92583
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种属 |
Human
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计算分子量 |
27.7 kDa
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表观分子量 |
30 kDa
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标签 |
N-Trx
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生物活性 |
Not validated for activity
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纯度 |
> 90% as determined by reducing SDS-PAGE.
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内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
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保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
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复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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背景 |
This antimicrobial gene is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 16. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for T lymphocytes, but not monocytes or granulocytes. The product of this gene binds to chemokine receptors CCR4 and CCR8. This chemokine plays important roles in T cell development in thymus as well as in trafficking and activation of mature T cells.
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Q1:重组蛋白有活性吗?可以做活性检测吗?
活性说明的见说明书bio-activity部分;如果说明书显示没有生物活性或则显示生物活性检测中,则说明这个蛋白没有生物活性数据,需要客户自己检测。
Q2:重组蛋白是否测序验证过?
在售重组蛋白默认不做测序分析/验证。
Q3:蛋白的运输条件是什么?
我们的蛋白有液体和冻干粉2种形式,一般来说冻干粉可以用冰袋、蓝冰、干冰运输;液体可以用蓝冰或干冰运输。
Q4:蛋白是通过什么方式纯化的?
一般通过纯化标签进行亲和纯化,常规有镍柱/His Tag、protein AG/Fc tag、标签抗体凝胶等。
Q5:蛋白可以用于细胞培养/注射小鼠吗?
内毒素含量小于0.1 ng/μg (1 EU/μg)的蛋白都可以用于细胞培养/注射小鼠。
Q6:蛋白发货形式可以是冻干粉吗?可以发液体吗?
默认按照说明书标注的形式发货;如果有特殊需求,需要根据蛋白情况确定。原则上,冻干粉蛋白可以发液体形式,液体蛋白不能发冻干粉形式。
Q7:蛋白Buffer里是否含有denaturants(变性剂)?
我们在售重组蛋白一般不含变性剂,具体见说明书的formulation信息。常见变性剂有:尿素和盐酸胍。
Q8:无标签蛋白纯化方式?
蛋白的纯化方式一般通过标签进行亲和纯化,无标签的蛋白是在纯化后进行了标签切除。
Q9:AF蛋白可以提供吗?
在售的重组蛋白都属于Animal component free protein。在售重组蛋白里不含任何直接来源与动物和人的制品:比如血清,血浆,全血,BSA,OVA等。
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