Recombinant Human WNT3A protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： P56704
| 别称 |
Protein Wnt-3a;WNT3A
|
| 表达系统 |
E.coli
|
| 序列 |
Ser19-Lys325
|
| 蛋白编码 |
P56704
|
| 种属 |
Human
|
| 计算分子量 |
33.7 kDa
|
| 表观分子量 |
38 kDa
|
| 标签 |
N-His
|
| 生物活性 |
Not validated for activity
|
| 纯度 |
> 95% as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
|
| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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| 背景 |
Wnt-3a is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes . Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta-catenin levels . As intracellular beta-catenin levels rise, beta-catenin binds to TCF/LEF transcription factors leading to expression of Wnt target genes.
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实验操作视频
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