Recombinant Human Zic3 protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： O60481
| 别称 |
ZIC3;Zinc finger protein 203;Zinc finger protein ZIC 3;Zinc finger protein of the cerebellum 3
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| 表达系统 |
E.coli
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| 序列 |
Met1-Val467
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| 蛋白编码 |
O60481
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| 种属 |
Human
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| 计算分子量 |
51.3 kDa
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| 表观分子量 |
52 kDa
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| 标签 |
N-His & C-His
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| 生物活性 |
Not validated for activity
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| 纯度 |
> 95% as determined by reducing SDS-PAGE.
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| 内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
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| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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| 背景 |
ZIC3, or zic family member 3, is a molecule that regulates early embryonic patterning in vertebrates. ZIC3 has putative roles in a number of developmental signalling pathways that have distinct roles in establishing the left-right axis. In addition, ZIC3 is also considered as target of MiR-564, a tumor suppressor in human lung cancer. Variants in the ZIC3 gene are rare, but have demonstrated their profound clinical significance in X-linked heterotaxy, affecting in particular male patients with abnormal arrangement of thoracic and visceral organs.
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实验操作视频
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