Recombinant MERS-CoV S-trimer Protein (R751S, C-6His)
Price:
Size:
50 μg
- 表达系统： HEK293 Cells
- 蛋白编码： AFS88936.1
- 应用性： ELISA
| 别称 |
MERS-CoV S glycoprotein;MERS-CoV S protein;MERS-CoV S-trimer Protein;MERS-CoV Spike glycoprotein
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| 表达系统 |
HEK293 Cells
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| 序列 |
Tyr18-Lys1294(R751S)
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| 蛋白编码 |
AFS88936.1
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| 种属 |
MERS
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| 计算分子量 |
145 kDa
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| 表观分子量 |
170-220 kDa
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| 标签 |
C-His
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| 生物活性 |
Immobilized MERS-CoV S-trimer Protein (R751S)-His(PKSV030287) at 5μg/ml (100 μl/well) can bind Human CD26-Fc(PKSH033696), The ED50 of Human CD26-Fc(PKSH033696) is 27.16 ng/ml.
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| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
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| 内毒素 |
Please contact us for more information.
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| 保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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| 运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
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| 制剂 |
Supplied as a 0.2 μm filtered solution of PBS, pH7.4.
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| 复溶方法 |
Not Applicable
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| 背景 |
The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
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实验操作视频
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