Recombinant Monomeric SA Protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： 6ZYT_AAA
| 别称 |
Monomeric SA;mSA
|
| 表达系统 |
E.coli
|
| 序列 |
Ala17-Lys130
|
| 蛋白编码 |
6ZYT_AAA
|
| 种属 |
Rhizavidin
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| 计算分子量 |
12.5 kDa
|
| 表观分子量 |
13-15 kDa
|
| 标签 |
N-His
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| 生物活性 |
Not validated for activity
|
| 纯度 |
> 95% as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
|
| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
|
| 背景 |
Forms a non-covalent specific complex with biotin. Monomeric streptavidin has unique structural and functional properties that can lead to development of new applications based on the well-known protein–ligand interaction. For example, it allows detection of biotinylated ligands while avoiding target aggregation caused by multivalent binding. Since clustering of biologically active molecules often results in functional changes, monomeric streptavidin can facilitate imaging and tracking studies in live cells by ensuring monovalent biotin binding.
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实验操作视频
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