Recombinant Mouse ADIPOQ Protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： Q60994-1
- 应用性： Immunogen
| 别称 |
30 kDa adipocyte complement related protein;30 kDa adipocyte complement-related protein;ACDC;ACRP30;ADIPO;Adipocyte;Adipocyte C1q and collagen domain containing protein;Adipocyte complement related 30 kDa protein;Adipocyte complement related protein of 30
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| 表达系统 |
E.coli
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| 序列 |
Glu18-Asn247
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| 蛋白编码 |
Q60994-1
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| 种属 |
Mouse
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| 计算分子量 |
24.9 kDa
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| 表观分子量 |
27.22 kDa
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| 标签 |
N-His
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| 生物活性 |
Not validated for activity
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| 纯度 |
> 85% as determined by reducing SDS-PAGE.
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| 内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
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| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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| 背景 |
Adiponectin (AdipoQ),an adipocyte-derived hormone,is one of the most abundant adipokines in the blood circulation. Adiponectin modulates a number of metabolic processes,including improving insulin sensitivity and anti-inflammatory activity. The role of AdipoQ in reproduction is not yet fully understood,but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans,including chicken testis,bovine ovary,and human placenta. Adiponectin exerts its effects by activating a range of different signaling molecules via binding to two transmembrane AdipoQ receptors,AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle,whereas AdipoR2 is predominantly expressed in the liver. AdipoQ May play a role in cell growth,angiogenesis and tissue remodeling by binding and sequestering various growth factors.
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Q1:重组蛋白有活性吗？可以做活性检测吗？
活性说明的见说明书bio-activity部分；如果说明书显示没有生物活性或则显示生物活性检测中，则说明这个蛋白没有生物活性数据，需要客户自己检测。
Q2:重组蛋白是否测序验证过？
在售重组蛋白默认不做测序分析/验证。
Q3:蛋白的运输条件是什么？
我们的蛋白有液体和冻干粉2种形式，一般来说冻干粉可以用冰袋、蓝冰、干冰运输；液体可以用蓝冰或干冰运输。
Q4:蛋白是通过什么方式纯化的？
一般通过纯化标签进行亲和纯化，常规有镍柱/His Tag、protein AG/Fc tag、标签抗体凝胶等。
Q5:蛋白可以用于细胞培养/注射小鼠吗？
内毒素含量小于0.1 ng/μg (1 EU/μg)的蛋白都可以用于细胞培养/注射小鼠。
Q6:蛋白发货形式可以是冻干粉吗？可以发液体吗？
默认按照说明书标注的形式发货；如果有特殊需求，需要根据蛋白情况确定。原则上，冻干粉蛋白可以发液体形式，液体蛋白不能发冻干粉形式。
Q7:蛋白Buffer里是否含有denaturants（变性剂）？
我们在售重组蛋白一般不含变性剂，具体见说明书的formulation信息。常见变性剂有：尿素和盐酸胍。
Q8:无标签蛋白纯化方式？
蛋白的纯化方式一般通过标签进行亲和纯化，无标签的蛋白是在纯化后进行了标签切除。
Q9:AF蛋白可以提供吗？
在售的重组蛋白都属于Animal component free protein。在售重组蛋白里不含任何直接来源与动物和人的制品：比如血清，血浆，全血，BSA，OVA等。
实验操作视频
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