Recombinant Mouse BGLAP Protein(Trx Tag)
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- 表达系统： E.coli
- 蛋白编码： P86546
| 别称 |
Bone Gamma-carboxyglutamate Protein;OT;Osteocalcin;BGP;Bglap;Bone Gla protein;Gamma-carboxyglutamic acid-containing protein
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| 表达系统 |
E.coli
|
| 序列 |
Tyr50-Ile95
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| 蛋白编码 |
P86546
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| 种属 |
Mouse
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| 计算分子量 |
25.1 kDa
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| 表观分子量 |
32 kDa
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| 标签 |
N-Trx
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| 生物活性 |
Not validated for activity
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| 纯度 |
> 95% as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
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| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis
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| 背景 |
This locus represents naturally occurring read-through transcription between the neighboring PMF1 (polyamine-modulated factor 1) and BGLAP (bone gamma-carboxyglutamate Gla protein) genes on chromosome 1. Alternative splicing results in multiple transcript variants encoding isoforms that share sequence identity with the upstream gene product, but they contain distinct C-termini due to frameshifts versus the downstream gene coding sequence. Recombinant human PMF1-BGLAP protein, fused to His-tag at N-terminus, was expressed in E.coli.
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实验操作视频
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