Recombinant Mouse CCL8/MCP-2 Protein (His Tag)
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- 表达系统： HEK293 Cells
- 蛋白编码： Q9Z121
| 别称 |
C-C motif chemokine 8;Ccl8;MCP-2;Mcp2;Monocyte chemoattractant protein 2;Monocyte chemotactic protein 2;Scya8;Small-inducible cytokine A8
|
| 表达系统 |
HEK293 Cells
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| 序列 |
Glu20-Pro97
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| 蛋白编码 |
Q9Z121
|
| 种属 |
Mouse
|
| 计算分子量 |
9.8 kDa
|
| 表观分子量 |
12 kDa
|
| 标签 |
C-His
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| 生物活性 |
Not validated for activity
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 20mM NaAc-HAc, 150mM NaCl, pH 4.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
Chemokine ligand 8 (CCL8,MCP-2), is a small secreted cytokine which belongs to the intercrine beta (chemokine CC) family. CCL8 Chemotactic factor attracts monocytes. It can bind heparin.CCL8 functions to activate different immune cells, including mast cells, eosinophils and basophils which are involved in allergic responses, monocytes, and T cells and NK cells which are involved in the inflammatory response. Its ability achieves by binding to different cell surface receptors termed chemokine receptors including CCR1, CCR2B and CCR5. It has been reported that CCL8 is a potent inhibitor of HIV-1 by virtue of its binding to CCR5 which is one of the major co-receptors for HIV-1.
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实验操作视频
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