SOW;Gsfsow;SCO;W;Bs;Fdc;Ssm;SCO1;SCO5;SOW3;CD117;c-KIT;Tr-kit;Gsfsco1;Gsfsco5;Gsfsow3;Mast/stem cell growth factor receptor Kit;Proto-oncogene c-Kit;SCFR;Tyrosine-protein kinase Kit;Sl;Kit; SCFR;CD117;c-KIT;Fdc;Gsfsco1;Gsfsco5;Gsfsow3;SCO1;SCO5;SOW3;Ssm;Tr-kit;KIT proto-oncogene receptor tyrosine kinase;PBT

Mammalian

Ser25-Pro527

P05532

Mouse

80.2 kDa

90 kDa

C-Fc

Not validated for activity

> 90% as determined by reducing SDS-PAGE.

< 1.0 EU/mg of the protein as determined by the LAL method

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

This product is provided as lyophilized powder which is shipped with ice packs.

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

C-Kit is a type 3 transmembrane receptor for MGF (mast cell growth factor , also known as stem cell factor). c-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. It belongs to the protein kinase superfamily , tyr protein kinase family , and CSF-1/PDGF receptor subfamily. C-Kit has tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase. Antibodies to c-Kit are widely used in immunohistochemistry to help distinguish particular types of tumor in histological tissue sections. It is used primarily in the diagnosis of GISTs. In GISTs , c-Kit staining is typically cytoplasmic , with stronger accentuation along the cell membranes. C-Kit antibodies can also be used in the diagnosis of mast cell tumors and in distinguishing seminomas from embryonal carcinomas. Mutations in the c-Kit gene are associated with gastrointestinal stromal tumors , mast cell disease , acute myelogenous leukemia , and piebaldism. Defects in KIT are a cause of acute myelogenous leukemia (AML). AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Note=Somatic mutations that lead to constitutive activation of KIT are detected in AML patients.