Recombinant Mouse CD27 Protein(His Tag)

Price:
- 表达系统: Mammalian
- 蛋白编码: P41272
别称 |
Tnfrsf;Tnfrsf7;CD27;T14;S152;TNFRSF7;CD27 antigen;CD27L receptor;S152;T-cell activation antigen CD27;Tp55;Tumor necrosis factor receptor superfamily member 7
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表达系统 |
Mammalian
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序列 |
Pro24-Arg182
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蛋白编码 |
P41272
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种属 |
Mouse
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计算分子量 |
17.4 kDa
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表观分子量 |
35-40 kDa
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标签 |
C-His
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生物活性 |
Not validated for activity
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纯度 |
> 90% as determined by reducing SDS-PAGE.
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内毒素 |
< 1.0 EU/mg of the protein as determined by the LAL method
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保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
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复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis
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背景 |
CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has an important role in the generation of T cell immunity and is an robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis.
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实验操作视频
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