Recombinant Mouse Ctla4 Protein(His Tag)（PDMM100100）|-Elabscience伊莱瑞特【官网】

Recombinant Mouse Ctla4 Protein(His Tag)

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货号：PDMM100100
说明书

说明书

Recombinant Mouse Ctla4 Protein(His Tag)

Price: ¥3600.00 ¥1200.00

Size:

100 μg 20 μg

表达系统： Mammalian
蛋白编码： P09793

别称	ALPS5;CD 152;CD;CD152;CD152 antigen;CD152 isoform;Celiac disease 3;CELIAC3;CTLA 4;CTLA-4;CTLA4;CTLA4;Cytotoxic T cell associated 4;Cytotoxic T lymphocyte antigen 4;Cytotoxic T lymphocyte associated 4;Cytotoxic T lymphocyte associated 4;soluble isoform;included;Cytotoxic T lymphocyte associated antigen 4;Cytotoxic T lymphocyte associated antigen 4 short spliced form;Cytotoxic T lymphocyte associated protein 4;Cytotoxic T lymphocyte associated serine esterase 4;Cytotoxic T lymphocyte protein 4;Cytotoxic T-lymphocyte protein 4;Cytotoxic T-lymphocyte-associated antigen 4;GRD4;GSE;ICOS;IDDM12;insulin-dependent diabetes mellitus 12;Ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4;OTTHUMP00000216623
表达系统	Mammalian
序列	Glu36-Asp161
蛋白编码	P09793
种属	Mouse
计算分子量	13.8 kDa
表观分子量	20-30 kDa
标签	C-His
生物活性	Not validated for activity
纯度	> 90% as determined by reducing SDS-PAGE.
内毒素	< 1.0 EU/mg of the protein as determined by the LAL method
保存条件	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
运输条件	This product is provided as lyophilized powder which is shipped with ice packs.
制剂	Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
复溶方法	It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
背景	Cytotoxic T-lymphocyte protein 4, also known as CTLA4 and CD152, is a single-pass type I membrane protein and a member of the immunoglobulin superfamily. It is the second member of the CD28 receptor family. The ligands or counterreceptors for these two proteins are the B7 family members, CD80 (B7-1) and CD86 (B7-2). CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may play an important role in their functions. CD152 or cytotoxic T lymphocyte antigen-4 (CTLA-4) is an essential receptor involved in the negative regulation of T cell activation. Because of its profound inhibitory role, CD152 has been considered a sound susceptible candidate in autoimmunity and a persuasive target for cancer immunotherapy. In particular, recent evidence suggests that CD152 is also important in the homeostasis and function of a population of suppressive cells, termed regulatory T cells (Treg).

SDS-PAGE analysis of Mouse Ctla4 Cd152 proteins, 2 μg/lane of Recombinant Mouse Ctla4 Cd152 proteins was resolved with an SDS-PAGE under reducing conditions, showing bands at 13.8KD

Q1:重组蛋白有活性吗？可以做活性检测吗？

活性说明的见说明书bio-activity部分；如果说明书显示没有生物活性或则显示生物活性检测中，则说明这个蛋白没有生物活性数据，需要客户自己检测。

Q2:重组蛋白是否测序验证过？

在售重组蛋白默认不做测序分析/验证。

Q3:蛋白的运输条件是什么？

我们的蛋白有液体和冻干粉2种形式，一般来说冻干粉可以用冰袋、蓝冰、干冰运输；液体可以用蓝冰或干冰运输。

Q4:蛋白是通过什么方式纯化的？

一般通过纯化标签进行亲和纯化，常规有镍柱/His Tag、protein AG/Fc tag、标签抗体凝胶等。

Q5:蛋白可以用于细胞培养/注射小鼠吗？

内毒素含量小于0.1 ng/μg (1 EU/μg)的蛋白都可以用于细胞培养/注射小鼠。

Q6:蛋白发货形式可以是冻干粉吗？可以发液体吗？

默认按照说明书标注的形式发货；如果有特殊需求，需要根据蛋白情况确定。原则上，冻干粉蛋白可以发液体形式，液体蛋白不能发冻干粉形式。