Recombinant Mouse G-CSF protein(N-His)
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- 表达系统： E.coli
- 蛋白编码： P09920
- 应用性： Cell culture
| 别称 |
OPGL;CD254;ODF (Osteoclast Differentiation Factor);TNFSF11;TRANCE (TNF-Related Activation-induced Cytokine);sRNAK Ligand;soluble Receptor Activator of NF-kB Ligand
|
| 表达系统 |
E.coli
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| 序列 |
Val 31-Ala 208
|
| 蛋白编码 |
P09920
|
| 种属 |
Mouse
|
| 计算分子量 |
19.8 kDa
|
| 表观分子量 |
17-25 kDa
|
| 标签 |
N-His
|
| 生物活性 |
Measure by its ability to induce proliferation in NFS-60 cells. The ED50 for this effect is <50 pg/mL. The specific activity of recombinant mouse G-CSF is > 2 x 107 IU/mg.
|
| 纯度 |
> 98 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 0.1 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from sterile PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Granulocyte colony-stimulating factor (G-CSF) is a growth factor and an essential cytokine which belongs to the IL-6 superfamily. Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. G-CSF binding to its receptor G-CSF-R which belongs to the cytokine receptor type I family depends on the interaction of alpha-helical motifs of the former and two fibronectin type III as well as an immunoglobulin-like domain of the latter. G-CSF is a cytokine that have been demonstrated to improve cardiac function and perfusion in myocardial infarction. And it was initially evaluated as a stem cell mobilizer and erythropoietin as a cytoprotective agent.
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实验操作视频
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