Recombinant Mouse Hepcidin/Hepc protein (TRX,His Tag)
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- 表达系统： E.coli
- 蛋白编码： Q9EQ21
| 别称 |
HEPC_MOUSE;Hamp;Hamp1;Hepc;Hepc1;Hepcidin
|
| 表达系统 |
E.coli
|
| 序列 |
Asp59-Thr83
|
| 蛋白编码 |
Q9EQ21
|
| 种属 |
Mouse
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| 计算分子量 |
22.6 kDa
|
| 表观分子量 |
25 kDa
|
| 标签 |
N-Trx-His
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| 生物活性 |
Not validated for activity
|
| 纯度 |
> 95% as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
|
| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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| 背景 |
Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes.
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实验操作视频
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