Recombinant Mouse HP1α protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： Q61686
| 别称 |
Antigen p25;CBX5;Chromobox 5;Epididymis luminal protein 25;HEL25;HP1 alpha;HP1A;Heterochromatin protein 1
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| 表达系统 |
E.coli
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| 序列 |
Met1-Ser191
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| 蛋白编码 |
Q61686
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| 种属 |
Mouse
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| 计算分子量 |
20.9 kDa
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| 表观分子量 |
25 kDa
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| 标签 |
N-His
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| 生物活性 |
Not validated for activity
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| 纯度 |
> 95% as determined by reducing SDS-PAGE.
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| 内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
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| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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| 背景 |
Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
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实验操作视频
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