Recombinant Mouse IL10 protein (His Tag)
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- 表达系统： HEK293 Cells
- 蛋白编码： P18893
| 别称 |
Interleukin;IL-;CSIF;If2a;Il-10;IL10A;TGIF;Interleukin-10;IL10;Cytokine synthesis inhibitory factor;N/A;CSIF;If2a;Il-10
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| 表达系统 |
HEK293 Cells
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| 序列 |
Met1-178Ser
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| 蛋白编码 |
P18893
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| 种属 |
Mouse
|
| 计算分子量 |
19.5 kDa
|
| 表观分子量 |
22 kDa
|
| 标签 |
C-His
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| 生物活性 |
Not validated for activity
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| 纯度 |
> 95% as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU/mg of the protein as determined by the LAL method
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
|
| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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| 背景 |
Mouse Il10 is the prototypic member of the IL-10 cytokine family, including IL-10, IL-19, IL-20, IL-22 (IL-TIF), IL-24 and IL-26. Many viruses encode viral members of the IL-10 family, such as Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV).Its main function is inhibiting the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. Although human and mouse IL-10 are 81% identical at the nucleotide and amino acid level, mouse IL-10 is species-specific and does not act on human cells. Interestingly, Human IL-10 is active on mouse cells.
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实验操作视频
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