Recombinant Mouse IL15RA/CD215 Protein (Fc Tag)
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- 表达系统： HEK293 Cells
- 蛋白编码： Q60819
- 应用性： Cell culture
| 别称 |
Interleukin-15 receptor subunit alpha;sIL-15 receptor subunit alpha;Il15ra;IL-15R&alpha
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| 表达系统 |
HEK293 Cells
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| 序列 |
Gly33-Lys205
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| 蛋白编码 |
Q60819
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| 种属 |
Mouse
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| 计算分子量 |
45.5 kDa
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| 表观分子量 |
60-90 kDa
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| 标签 |
C-Fc
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| 生物活性 |
Measured by its ability to block human IL-15-induced proliferation of CTLL‑2 mouse cytotoxic T cells. The ED50 for this effect is 0.5-2 ng/ml.
|
| 纯度 |
> 90 % as determined by reducing SDS-PAGE.
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| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
Mouse interleukin-15 receptor subunit alpha, also known as Il15ra, is a high-affinity receptor for interleukin-15. Il15ra associates as a heterotrimer with the IL-2 receptor beta and gamma subunits (Common gamma chain, or gamma c) to initiate signal transduction. It can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells. Il15ra is expressed in special cells including a wide variety of Tand B cells and non-lymphoid cells. Human Il15ra shares 45% amino acid sequence homology with the mouse form of the receptor. Eight isoforms of IL-15 R alpha mRNA have been identified, resulting from alternative splicing events involving different exons.
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实验操作视频
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