Recombinant Mouse IL7RA/CD127 Protein (Fc Tag)
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- 表达系统： HEK293 Cells
- 蛋白编码： P16872
- 应用性： BLI
| 别称 |
CD127;IL-7 receptor subunit alpha;IL-7R subunit alpha;IL-7RA;IL-7Ralpha;IL-7R-alpha;Interleukin-7 receptor subunit alpha;Il7r
|
| 表达系统 |
HEK293 Cells
|
| 序列 |
Glu21-Asp239
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| 蛋白编码 |
P16872
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| 种属 |
Mouse
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| 计算分子量 |
52.1 kDa
|
| 表观分子量 |
61-75 kDa
|
| 标签 |
C-Fc
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| 生物活性 |
Loaded Human IL-7-His on HIS1K Biosensor, can bind Mouse IL-7RA-Fc with an affinity constant of 5.91 nM as determined in BLI assay.
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 10% Sucrose, 3% Glycine, 100mM NaCl, 0.1mM EDTA, 0.05% Tween 80, pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
Interleukin 7 Receptor alpha (IL-7Rα), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival. IL-7Rα is majorly expressed on T cells and their precursors, and early in B cell development as well, prior to the appearance of surface IgM. Dynamic regulation of IL-7Rα is important for the generation of appropriate immune responses.
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实验操作视频
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