Recombinant Mouse IL-3 Protein(Trx Tag)
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- 表达系统： E.coli
- 蛋白编码： P01586
| 别称 |
Interleukin;Hematopoietic Growth Factor;Interleukin-3;Mast Cell Growth Factor;MCGF;MCSF;MULTI-CSF;Multipotential Colony-Stimulating Factor;P-Cell Stimulating Factor;P-Cell-Stimulating Factor;Csfmu;Il-3;Il3
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| 表达系统 |
E.coli
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| 序列 |
Ala27-Cys166
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| 蛋白编码 |
P01586
|
| 种属 |
Mouse
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| 计算分子量 |
35.3 kDa
|
| 表观分子量 |
35 kDa
|
| 标签 |
N-Trx
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| 生物活性 |
Not validated for activity
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| 纯度 |
> 90% as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
|
| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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| 背景 |
IL3 (interleukin 3), also known as IL-3, is a potent growth-promoting cytokine that belongs to the IL-3 family. IL3/IL-3 also belongs to the group of interleukins. Interleukins are produced by a wide variety of body cells. The function of the immune system depends in a large part on interleukins, and rare deficiencies of a number of them have been described, all featuring autoimmune diseases or immune deficiency. The majority of interleukins are synthesized by helper CD4+ T lymphocytes, as well as throμgh monocytes, macrophages, and endothelial cells. They promote the development and differentiation of T, B, and hematopoietic cells. IL3/IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation, and apoptosis. IL3/IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
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实验操作视频
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