Recombinant Mouse IL-7 protein(His Tag)
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- 表达系统： E.coli
- 蛋白编码： P10168
- 应用性： Cell culture
| 别称 |
Lymphopoietin;IL-7 interleukin;IL-7 interleukin-7;Interleukin-7;Lymphopoietin -1;PBGF;Il-7;Il7
|
| 表达系统 |
E.coli
|
| 序列 |
Glu 26-Ile 154
|
| 蛋白编码 |
P10168
|
| 种属 |
Mouse
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| 计算分子量 |
15.8 kDa
|
| 表观分子量 |
16 kDa
|
| 标签 |
C-His
|
| 生物活性 |
Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBMC).The ED50 for this effect is <0.2 ng/mL.The specific activity of recombinant mouse IL-7 is > 5 x 106 IU/mg.
|
| 纯度 |
> 98 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 0.1 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from sterile PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
IL7, also known as interleukin 7, is a hematopoietic growth factor that belongs to the IL-7/IL-9 family. It is secreted by stromal cells in the bone marrow and thymus. IL7 stimulates the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. IL7 and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. It is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRß) during early T cell development. IL7 can be produced locally by intestinal epithelial and epithelial goblet cells and may serve as a regulatory factor for intestinal mucosal lymphocytes.
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实验操作视频
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