Recombinant Mouse Interleukin-2/IL-2 Protein
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- 表达系统： E.coli
- 蛋白编码： P04351
- 应用性： Cell culture
| 别称 |
POIL;OTTHUMP;Aldesleukin;IL 2;Interleukin 2;Involved in regulation of T cell clonal expansion;Lymphokine;OTTHUMP00000164090;POIL2;T Cell Growth Factor;T-cell growth facter;Interleukin-2;IL2;TCGF;T-cell growth factor;Il-2
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| 表达系统 |
E.coli
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| 序列 |
Ala21-Gln169
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| 蛋白编码 |
P04351
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| 种属 |
Mouse
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| 计算分子量 |
17.4 kDa
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| 表观分子量 |
17 kDa
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| 标签 |
None
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| 生物活性 |
Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells.The specific activity of Recombinant Mouse IL-2 is ≥1×107 IU/mg.
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| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
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| 内毒素 |
< 0.01 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 20mM Sodium Citrate, 0.2% Tween 80, pH 3.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
Interleukin 2 (IL 2), also termed T-cell growth factor, is a member of the cytokine family which includes IL-4, IL-7, IL-9, IL-15 and IL-21. Each member of this family has a four alpha helix bundle. IL-2 signals through the IL-2 receptor, a complex consisting of tree subunits, termed alpha, beta and gamma. The IL-2 R gamma is shared by cytokine receptors of all members of cytokine family. Mature mouse IL-2 shares 56% and 73% aa sequence identity with human and rat IL-2, respectively. IL-2 is produced by CD4+ T cell, CD8+ T cells, gamma δ T cells, B cells, dendritic cells and eosinophils, and plays a vital role in key function of the immune system, tolerance and immunity, primarily via its potent stimulatory activity for T cells.Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity.
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实验操作视频
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