Recombinant Mouse Interleukin-5/IL-5 (C-6His)
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- 表达系统： HEK293 Cells
- 蛋白编码： P04401
| 别称 |
Interleukin;B-cell differentiation factor I;E-CSF;EDF;ESGF;PRL;Prolactin;Interleukin-5;BCGF-II;TRF;Il5;B-cell growth factor II;Cytotoxic T-lymphocyte inducer;Eosinophil differentiation factor;T-cell replacing factor;Il-5
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| 表达系统 |
HEK293 Cells
|
| 序列 |
Met21-Gly133
|
| 蛋白编码 |
P04401
|
| 种属 |
Mouse
|
| 计算分子量 |
13.9 kDa
|
| 表观分子量 |
14-26 kDa
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| 标签 |
C-His
|
| 生物活性 |
Not validated for activity
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
IL-5 is expressed in eosinophils, NK cells, TC2 CD8+ T cells, mast cells, CD45+ CD4+ T cells, gamma delta T cells and IL-1 beta activated endothelial cells. IL-5 acts as a growth and differentiation factor for both B cells and eosinophils. Relative to B cells, IL-5 appears to induce the differentiation of activated conventional B-2 cells into Ig-secreting cells. In addition, it induces the growth of B-1 progenitors as well as IgM production by B-1 cells.IL-5 appears to perform a number of functions on eosinophils. These include the down modulation of Mac-1,the upregulation of receptors for IgA and IgG,the stimulation of lipid mediator (leukotriene C4 and PAF) secretion and the induction of granule release.IL-5 also promotes the growth and differentiation of eosinophils.
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实验操作视频
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