Recombinant Mouse M-CSF protein(His Tag)
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- 表达系统： E.coli
- 蛋白编码： P07141
- 应用性： Cell culture
| 别称 |
Ath;Ath-;Ath-1;Ath1;CD134;CD134L;OX-40L;OX4;TNFSF4;TXGP1;Tnlg2b;Txgp;Txgp1l;gp3;gp34
|
| 表达系统 |
E.coli
|
| 序列 |
Lys32-Pro187
|
| 蛋白编码 |
P07141
|
| 种属 |
Mouse
|
| 计算分子量 |
19.0 kDa
|
| 表观分子量 |
17-25 kDa
|
| 标签 |
C-His
|
| 生物活性 |
Measure by its ability to induce proliferation in NFS-60 cells.The ED50 for this effect is <2 ng/mL.The specific activity of recombinant mouse M-CSF is approximately >5 x 105 IU/mg.
|
| 纯度 |
> 98 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 0.1 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from sterile PBS,pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Macrophage colony-stimulating factor 1（M-csf）is a single-pass type I membrane protein . It is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. M-CSF affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. The role of M-CSF is not only restricted to the monocyte/macrophage cell lineage. By interacting with its membrane receptor, M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy.
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实验操作视频
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