Recombinant Mouse Ngfb Protein(His Tag)（PDEM100338）|-Elabscience伊莱瑞特【官网】

Recombinant Mouse Ngfb Protein(His Tag)

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货号：PDEM100338
说明书

说明书

Price: ¥3600.00 ¥1200.00

Size:

100 μg 20 μg

表达系统： E.coli
蛋白编码： P01139

别称	Beta-nerve growth factor;Beta-NGF;NGF
表达系统	E.coli
序列	Ser122-Gly241
蛋白编码	P01139
种属	Mouse
计算分子量	13.1 kDa
表观分子量	13 kDa
标签	N-His
生物活性	Not validated for activity
纯度	> 95% as determined by reducing SDS-PAGE.
内毒素	< 10 EU/mg of the protein as determined by the LAL method
保存条件	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
运输条件	This product is provided as lyophilized powder which is shipped with ice packs.
制剂	Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
复溶方法	It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
背景	NGF is the first member discovered in the Neurotrophin family, which includes brain-derived neurotrophic factor(BDNF), neurotrophin-3(NT-3), and neurotrophin-4(NT-4). These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Mouse beta-NGF is a homodimer of two 120 amino acid polypeptides. It shares approximately 90% homology at the amino acid level with human beta-NGF and 95.8% with rat beta-NGF. NGF signaling has been shown to play an important role in neuroprotection and repair. β-NGF acts as a growth and differentiation factor for B lymphocytes, and enhances B-cell survival. It is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems.

SDS-PAGE analysis of Mouse Ngfb proteins,2μg/lane of Recombinant Mouse Ngfb proteins,was resolved with SDS-PAGE under reducing conditions,showing bands at 13 KD

Q1:重组蛋白有活性吗？可以做活性检测吗？

活性说明的见说明书bio-activity部分；如果说明书显示没有生物活性或则显示生物活性检测中，则说明这个蛋白没有生物活性数据，需要客户自己检测。

Q2:重组蛋白是否测序验证过？

在售重组蛋白默认不做测序分析/验证。

Q3:蛋白的运输条件是什么？

我们的蛋白有液体和冻干粉2种形式，一般来说冻干粉可以用冰袋、蓝冰、干冰运输；液体可以用蓝冰或干冰运输。

Q4:蛋白是通过什么方式纯化的？

一般通过纯化标签进行亲和纯化，常规有镍柱/His Tag、protein AG/Fc tag、标签抗体凝胶等。

Q5:蛋白可以用于细胞培养/注射小鼠吗？

内毒素含量小于0.1 ng/μg (1 EU/μg)的蛋白都可以用于细胞培养/注射小鼠。

Q6:蛋白发货形式可以是冻干粉吗？可以发液体吗？

默认按照说明书标注的形式发货；如果有特殊需求，需要根据蛋白情况确定。原则上，冻干粉蛋白可以发液体形式，液体蛋白不能发冻干粉形式。