Recombinant Mouse Prostatic Acid Phosphatase Protein(Fc Tag)-Elabscience伊莱瑞特【官网】

Recombinant Mouse Prostatic Acid Phosphatase Protein(Fc Tag)

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货号：PDMM100101
说明书

说明书

Recombinant Mouse Prostatic Acid Phosphatase Protein(Fc Tag)

Price: ¥3600.00 ¥1200.00

Size:

100 μg 20 μg

表达系统： Mammalian
蛋白编码： Q8CE08

别称	5'-NT;5'-nucleotidase;Acid phosphatase prostate;ACP 3;ACP3;acpP;Ecto-5'-nucleotidase;PAP;PPAP;Prostatic acid phosphatase;Prostatic acid phosphotase;Thiamine monophosphatase;TMPase
表达系统	Mammalian
序列	Met1-Asn381aa
蛋白编码	Q8CE08
种属	Mouse
计算分子量	66.8 kDa
表观分子量	80 kDa
标签	C-Fc
生物活性	Not validated for activity
纯度	> 90% as determined by reducing SDS-PAGE.
内毒素	< 1.0 EU/mg of the protein as determined by the LAL method
保存条件	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
运输条件	This product is provided as lyophilized powder which is shipped with ice packs.
制剂	Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
复溶方法	It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
背景	ACPP (Acid phosphatase, prostate, also PAP and ACP3) is a 48-52 kDa glycoprotein member of the histidine acid phosphatase family of enzymes. It exists as a 95-100 kDa nondisulfide-linked homodimer that hydrolyzes phosphate esters under low pH to generate free phosphate. ACPP is expressed by prostate epithelium and pain-detecting spinal cord neurons. In the spinal cord, ACPP dephosphorylates AMP. This generates adenosine which acts as a strong analgesic agent. Mature Human ACPP is 354 amino acids (aa) in length (aa 33-386). It contains one histidine phosphatase domain (aa 34-332), plus a nucleophile acceptor site at His44, and a proton donor site at Asp290. There are two potential alternative splice variants. One shows a deletion of aa 153-185, while another is transmembrane (previously called TMPase) and shows a 38 aa substitution for the C-terminal seven amino acids. Over aa 33-379, Human ACPP shares 84% aa identity with an Mouse ACPP.

SDS-PAGE analysis of Mouse Prostatic Acid Phosphatase/PPAP proteins, 2 μg/lane of Recombinant Mouse Prostatic Acid Phosphatase/PPAP proteins was resolved with an SDS-PAGE under reducing conditions, showing bands at 66.8KD