Recombinant Mouse TNF-α protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： P06804
| 别称 |
Cachectin;DIF;Membrane Form;TNF-Alpha;TNF-a;TNF-alpha;TNFSF2;TNFalpha;Tnf;Tnfa;Tnfsf1a;Tnfsf2;Tumor Necrosis Factor;Tumor Necrosis Factor Ligand Superfamily Member 2
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| 表达系统 |
E.coli
|
| 序列 |
Leu80–Leu235
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| 蛋白编码 |
P06804
|
| 种属 |
Mouse
|
| 计算分子量 |
17.1 kDa
|
| 表观分子量 |
17 kDa
|
| 标签 |
N-His
|
| 生物活性 |
Not validated for activity
|
| 纯度 |
> 95% as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
|
| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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| 背景 |
Tumor Necrosis Factor (TNF) is a member of the Tumor Necrosis Factor family. TNF exists as a homotrimer and interacts with SPPL2B. TNF is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. TNF is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes throught its effects on insulin resistance and fatty acid metabolism.
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实验操作视频
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