4 1BB;4 1BB ligand receptor;4-1BB ligand receptor;4-1BB Ligand Receptor T Cell;4-1BB;mouse;homolog of;Antigen 4-1BB Homolog;CD 137;CD137;CD137 antigen;CDw137;HLDA VI;Homolog of mouse 4 1BB;ILA;induced by lymphocyte activation;ILA;Induced by lymphocyte activation;Interleukin activated receptor homolog of mouse Ly63;Ly63;mouse;homolog of;MGC2172;OTTHUMP00000044294;Receptor protein 4 1BB;T cell antigen 4 1BB homolog;T cell antigen ILA;T-cell antigen 4-1BB homolog;T-cell antigen ILA;TNF receptor superfamily member 9;TNFRSF9;TNR9;Tumor necrosis factor receptor superfamily member 9

Mammalian

Val24-Leu187

P20334

Mouse

17.9 kDa

30-35 kDa

C-His

Not validated for activity

> 90% as determined by reducing SDS-PAGE.

< 1.0 EU/mg of the protein as determined by the LAL method

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

This product is provided as lyophilized powder which is shipped with ice packs.

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4+ and CD8+ T cells, and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. Upon ligand binding, 4-1BB is associated with an the tumor necrosis factor receptor–associated factors (TRAFs), the adaptor protein which mediates downstream signaling events including the activation of NF-kappaB and cytokine production. 4-1BB signaling either by binding to 4-1BBL or by antibody ligation delivers signals for T-cell activation and growth, as well as monocyte proliferation and B-cell survival, and plays an important role in the amplification of T cell-mediated immune responses. In addition, CD137 and CD137L are expressed in different human primary tumor tissues, suggesting that they may influence the progression of tumors. Crosslinking of CD137 on activated T cells has shown promise in enhancing anti-tumor immune responses in murine models, and agonistic anti-CD137 antibodies are currently being tested in phase I clinical trials. Soluble forms of CD137 (sCD137) are generated by differential splicing. sCD137 can bind to CD137 ligand to antagonize the costimulatory activities of the membrane-bound CD137 and reduce T cell proliferation and IL-2 secretion.