Recombinant Rabbit TNF-alpha/TNFSF2/TNFa Protein
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- 表达系统： E.coli
- 蛋白编码： P04924
- 应用性： Cell culture
| 别称 |
Cachectin;TNF;TNF-Alpha;TNF-a;TNFA;TNFSF2;Tumor Necrosis Factor;Tumor Necrosis Factor Ligand Superfamily Member 2
|
| 表达系统 |
E.coli
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| 序列 |
Val78-Leu235
|
| 蛋白编码 |
P04924
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| 种属 |
Rabbit
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| 计算分子量 |
17.6 kDa
|
| 表观分子量 |
18 kDa
|
| 标签 |
None
|
| 生物活性 |
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 3. 5 pg/ml.
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Tumor necrosis factor alpha (TNFα) is the prototypic ligand of the TNF superfamily. TNFα forms a homotrimer and functions by activating two types of receptors TNF-R1 (TNF receptor type 1,p55R) and TNF-R2 (TNF receptor type 2,p75R). TNFα is a pleiotropic cytokine that is capable to promote inflammation, to induce apoptotic cell death, and to inhibit tumorigenesis and viral replication. TNFα is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.
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A label-free AuNPs-based ultrasensitive plasmonic ELISA by alkaline phosphatase and ferrous ion-initiated cascade amplification
IF: 8.400Journal： SENSORS AND ACTUATORS B-CHEMICAL (2023)
实验操作视频
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