Recombinant Rabbit TNF-α protein (His Tag)

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- 表达系统: E.coli
- 蛋白编码: P04924
别称 |
Cachectin;TNF;TNF-Alpha;TNF-a;TNFA;TNFSF2;Tumor Necrosis Factor
|
表达系统 |
E.coli
|
序列 |
Leu80-Leu235
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蛋白编码 |
P04924
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种属 |
Rabbit
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计算分子量 |
17.1 kDa
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表观分子量 |
18 kDa
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标签 |
N-His
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生物活性 |
Not validated for activity
|
纯度 |
> 95% as determined by reducing SDS-PAGE.
|
内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
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保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
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复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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背景 |
Tumor necrosis factor alpha (TNFα) is the prototypic ligand of the TNF superfamily. TNFα forms a homotrimer and functions by activating two types of receptors TNF-R1 (TNF receptor type 1,p55R) and TNF-R2 (TNF receptor type 2,p75R). TNFα is a pleiotropic cytokine that is capable to promote inflammation, to induce apoptotic cell death, and to inhibit tumorigenesis and viral replication. TNFα is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.
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实验操作视频
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