Recombinant Rat CD73/NT5E Protein (His Tag)
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- 表达系统： HEK293 Cells
- 蛋白编码： P21588
| 别称 |
5'-Nucleotidase;CD73;E5NT;NT5E;Nucleotidase;ecto-5'-nucleotidase
|
| 表达系统 |
HEK293 Cells
|
| 序列 |
Trp29-Lys549
|
| 蛋白编码 |
P21588
|
| 种属 |
Rat
|
| 计算分子量 |
59.0 kDa
|
| 表观分子量 |
70-90 kDa
|
| 标签 |
C-His
|
| 生物活性 |
Not validated for activity
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
|
| 运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
|
| 制剂 |
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH 7.5.
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| 复溶方法 |
Not Applicable
|
| 背景 |
CD73, also known as Ecto-5'-nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor. The enzyme is expressed by most cell types. The 5 ’ -Nucleotidase activity of CD73 converts extracellular nucleoside-5 ’ -monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
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实验操作视频
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