Recombinant Rat IL-6 Protein
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- 表达系统： HEK293 Cells
- 蛋白编码： P20607
| 别称 |
BSF;IFNB;BSF2;CDF;HGF;HSF;IFNB2;Interleukin-6;Il6;Il 6;Interleukin 6;Il-6
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| 表达系统 |
HEK293 Cells
|
| 序列 |
Met1-Thr211
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| 蛋白编码 |
P20607
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| 种属 |
Rat
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| 计算分子量 |
24 kDa
|
| 表观分子量 |
25-33 kDa
|
| 标签 |
C-His
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| 生物活性 |
Not validated for activity
|
| 纯度 |
> 95% as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU/mg of the protein as determined by the LAL method
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
|
| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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| 背景 |
Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.
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A label-free AuNPs-based ultrasensitive plasmonic ELISA by alkaline phosphatase and ferrous ion-initiated cascade amplification
IF: 8.400Journal： SENSORS AND ACTUATORS B-CHEMICAL (2023)
实验操作视频
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