Recombinant Rat MMP-12 protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： Q63341
| 别称 |
Macrophage metalloelastase;MME;MMP-12;Matrix metalloproteinase-12;Mme;Mmel;Mmp12
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| 表达系统 |
E.coli
|
| 序列 |
Ala286-Phe463
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| 蛋白编码 |
Q63341
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| 种属 |
Rat
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| 计算分子量 |
19.5 kDa
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| 表观分子量 |
24 kDa
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| 标签 |
N-His
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| 生物活性 |
Not validated for activity
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| 纯度 |
> 95% as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
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| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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| 背景 |
Matrix metalloproteinase-12 (MMP12) is a secreted protein.It contains 4 hemopexin repeats and belongs to the peptidase M10A family. MMP12 may be involved in tissue injury and remodeling and have significant elastolytic activity. It can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
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实验操作视频
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