Recombinant Rat PPAR-α protein (His Tag)

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- 表达系统: E.coli
- 蛋白编码: P37230
别称 |
PPAR alpha;PPAR-alpha;PPARA;PPARMGC2452;PPARalpha
|
表达系统 |
E.coli
|
序列 |
Ser280-Leu433
|
蛋白编码 |
P37230
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种属 |
Rat
|
计算分子量 |
16.8 kDa
|
表观分子量 |
18 kDa
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标签 |
N-His
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生物活性 |
Not validated for activity
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纯度 |
> 95% as determined by reducing SDS-PAGE.
|
内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
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保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
|
复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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背景 |
Peroxisome Proliferator-Activated Receptor alpha (PPAR alpha, NR1C1) is a member of the Nuclear Receptor superfamily. PPAR alpha exhibits the highest affinity with unsaturated fatty acids, and linolenic acids. PPAR alpha is expressed in brown fat, liver, kidney, heart, mucosa of the stomach and duodenum, retina, adrenal gland, skeletal muscle, pancreatic islets and smooth muscle cells. PPAR alpha plays important roles in lipid and glucose metabolism, and has been implicated in obesity-related metabolic diseases such as hyperlipidemia, insulin resistance, and coronary artery disease. Three members have been identified and are called PPAR alpha, δ, and lambda. Retinoid X Receptor (RXR) is an obligate partner for PPAR.
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实验操作视频
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